Description
Anti-dsDNA is an enzyme immunoassay for the quantitative determination of IgG antibodies to dsDNA. The antibodies of the calibrators, control and
diluted patient samples react with dsDNA
immobilized on the solid phase of microtiter plates.
Highly purified dsDNA coated on the microtiter
plate guarantees the specific binding of dsDNA IgG
antibodies of the specimen under investigation.
Following an incubation period of 60 min at room
temperature (RT), unbound sample components
are removed by a wash step. The bound IgG antibodies react specifically with
anti-human-IgG conjugated to horseradish
peroxidase (HRP). Within the incubation period of
30 min at RT, excessive conjugate is separated from
the solid-phase immune complexes by the following
wash step. HRP converts the colorless substrate solution of
3,3’,5,5’-tetramethylbenzidine (TMB) added into a
blue product. The enzyme reaction is stopped by
dispensing an acidic solution into the wells after 15
min at room temperature turning the solution from
blue to yellow.
