Description
The Cortisol is a solid phase competitive ELISA. The samples and Cortisol enzyme
conjugate are added to the wells coated with anti-Cortisol monoclonal antibody. Cortisol in the
patient’s sample competes with a Cortisol enzyme conjugate for binding sites. Unbound Cortisol
and Cortisol enzyme conjugate is washed off by washing buffer. Upon the addition of the
substrate, the intensity of color is inversely proportional to the concentration of Cortisol in the
samples. A standard curve is prepared relating color intensity to the concentration of the Cortisol.
